Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp12783-Ser13467.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CA125, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA. See testing image for detail.
CA125
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Poids moléculaire
76.17 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.