PVRL1 Protein (His tag)

N° du produit ABIN7321172

Détail du produit

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Type de proteíne: Recombinant

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette PVRL1 protéine est marqué à la His tag.

Fonction: Recombinant Rat CD111/Nectin-1 Protein (His Tag)

Séquence: Met1-Ala354

Attributs du produit: A DNA sequence encoding the rat PVRL1 (XP_236210.3) (Met1-Ala354) was expressed, fused with a polyhistidine tag at the C-terminus.

Pureté: > 97 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Autre désignation: CD111/Nectin-1 (PVRL1 Produits)

Synonymes: CD111 Protein, CLPED1 Protein, ED4 Protein, HIgR Protein, HVEC Protein, OFC7 Protein, PRR Protein, PRR1 Protein, PVRR Protein, PVRR1 Protein, SK-12 Protein, nectin-1 Protein, prr1 Protein, PVRL1 Protein, pvrl1 Protein, AI835281 Protein, AW549174 Protein, Cd111 Protein, HveC Protein, cd111 Protein, cd112 Protein, clped1 Protein, ed4 Protein, higr Protein, hveb Protein, hvec Protein, nectin-2 Protein, ofc7 Protein, prr Protein, prr2 Protein, pvrl2 Protein, pvrl2.L Protein, pvrr Protein, pvrr1 Protein, pvrr2 Protein, sk-12 Protein, nectin cell adhesion molecule 1 Protein, nectin-1 Protein, Poliovirus receptor-related protein 1 Protein, poliovirus receptor-related 1 (herpesvirus entry mediator C) Protein, nectin cell adhesion molecule 2 L homeolog Protein, NECTIN1 Protein, LOC100145553 Protein, pvrl1 Protein, nectin1 Protein, Nectin1 Protein, PVRL1 Protein, nectin2.L Protein

Sujet:

Background: Poliovirus receptor-related 1 (herpesvirus entry mediator C, nectin-1, CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

Synonym: PVRL1

Poids moléculaire: 37.6 kDa

NCBI Accession: XP_236210

Pathways: Cell-Cell Junction Organization