REG4 Protein (His tag)

N° du produit ABIN7320368

Détail du produit

Antigène: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette REG4 protéine est marqué à la His tag.

Fonction: Recombinant Mouse REG4/RELP Protein (His Tag)(Active)

Séquence: Met 1-Thr 157

Attributs du produit: A DNA sequence encoding mouse REG4 (NP_080604.2) (Met 1-Thr 157) was expressed with the polyhistide tag at the C-terminus.

Pureté: > 95 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 10E4 cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), >70% cell will adhere after 60 minutes at 37°C.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Autre désignation: REG4/RELP (REG4 Produits)

Synonymes: GISP Protein, REG-IV Protein, RELP Protein, 2010002L15Rik Protein, regenerating family member 4 Protein, regenerating islet-derived family, member 4 Protein, Reg4 Protein, REG4 Protein

Sujet:

Background: Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

Synonym: 2010002L15Rik,GISP,RELP

Poids moléculaire: 17.5 kDa

NCBI Accession: NP_080604