SFRP1 Protein (His tag)

N° du produit ABIN7317804

Détail du produit

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette SFRP1 protéine est marqué à la His tag.

Fonction: Recombinant Human sFRP1/SARP2 Protein (His Tag)(Active)

Séquence: Met 1-Lys 314

Attributs du produit: A DNA sequence encoding the human sFRP1 (NP_003003.3) (Met 1-Lys 314) was expressed with a C-terminal polyhistidine tag.

Pureté: > 97 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5-10 μg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Autre désignation: sFRP1/SARP2 (SFRP1 Produits)

Synonymes: FRP Protein, FRP-1 Protein, FRP1 Protein, FrzA Protein, SARP2 Protein, 2210415K03Rik Protein, AW011917 Protein, AW107218 Protein, AW742929 Protein, sFRP-1 Protein, CSFRP1 Protein, SFRP1 Protein, XfrzA Protein, frp Protein, frp-1 Protein, frp1 Protein, frzA Protein, sarp2 Protein, sfrp1 Protein, LOC100231868 Protein, Srfp1a Protein, wu:fd19b09 Protein, secreted frizzled related protein 1 Protein, secreted frizzled-related protein 1 Protein, Secreted frizzled-related protein 1 Protein, secreted frizzled-related protein 1a Protein, secreted frizzled-related protein 1b Protein, SFRP1 Protein, Sfrp1 Protein, sfrp1 Protein, sfrp1a Protein, sfrp1b Protein

Sujet:

Background: Secreted frizzled-related protein 1, also known as sFRP1, is a 35 kDa prototypical member of the SFRP family. SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. The deregulation of this critical developmental pathway occurs in several human tumor entities. Mouse sFRP1 is highly expressed in kidney and embryonic heart, as well as in the eye, where it is principally localized to the ciliary body and the lens epithelium.

Synonym: FRP;FRP-1;FRP1;FrzA;SARP2

Poids moléculaire: 34 kDa

NCBI Accession: NP_003003

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation