TFPI2 Protein (His tag)

N° du produit ABIN7198257

Détail du produit

Antigène: TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette TFPI2 protéine est marqué à la His tag.

Fonction: Recombinant Human TFPI2 Protein (His Tag)(Active)

Séquence: Met 1-Lys 213

Attributs du produit: A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

Autre désignation: TFPI2 (TFPI2 Produits)

Synonymes: MGC68843 Protein, TFPI2 Protein, MGC108301 Protein, si:ch211-262k23.2 Protein, tfpi2 Protein, PP5 Protein, REF1 Protein, TFPI-2 Protein, AV000670 Protein, PP5/TFPI-2 Protein, tissue factor pathway inhibitor 2 L homeolog Protein, tissue factor pathway inhibitor 2 Protein, tfpi2.L Protein, TFPI2 Protein, tfpi2 Protein, Tfpi2 Protein

Sujet:

Background: Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.

Synonym: Tissue Factor Pathway Inhibitor 2, TFPI-2, Placental Protein 5, PP5, TFPI2

Poids moléculaire: 23.2 kDa

NCBI Accession: NP_006519