HCoV-OC43 N
(Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
Type de proteíne
Recombinant
Origine
Human Coronavirus OC43 (HCoV-OC43)
Source
Escherichia coli (E. coli)
Purification/Conjugué
Cette HCoV-OC43 N protéine est marqué à la His tag.
Fonction
Recombinant Human coronavirus (HCoV-OC43) Nucleoprotein / NP Protein (His Tag)
Séquence
Met1-Ile448
Attributs du produit
A DNA sequence encoding the Human coronavirus (HCoV-OC43) Nucleoprotein (YP_009555245.1) (Met1-Ile448) was expressed with a polyhistide tag at the N-terminus.
HCoV-OC43 N
Origine: Human Coronavirus OC43 (HCoV-OC43)
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 90 % as determined by reducing SDS-PAGE.
HCoV-OC43 N
Origine: Human Coronavirus OC43 (HCoV-OC43)
Hôte: Escherichia coli (E. coli)
Recombinant
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Please refer to the printed manual for detailed information.
Buffer
Lyophilized from sterile 40 mM PB, 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Antigène
HCoV-OC43 N
(Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.