TPBG Protein (AA 32-355) (His-Avi Tag,Biotin)

N° du produit ABIN7273785

Détail du produit

Antigène: TPBG (Trophoblast Glycoprotein (TPBG))

Type de proteíne: Recombinant

Attributs du protein: AA 32-355

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette TPBG protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human TPBG/5T4 Protein

Séquence: Ser32-Ser355

Attributs du produit: Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: TPBG (Trophoblast Glycoprotein (TPBG))

Autre désignation: TPBG (TPBG Produits)

Synonymes: 5T4 Protein, 5T4AG Protein, M6P1 Protein, AW495680 Protein, TPBG Protein, tpbg Protein, trophoblast glycoprotein Protein, trophoblast glycoprotein b Protein, TPBG Protein, Tpbg Protein, tpbgb Protein, tpbg Protein

Sujet: The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Poids moléculaire: 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.