ICOSLG Protein (His-Avi Tag,Biotin)

N° du produit ABIN7273968

Détail du produit

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ICOSLG protéine est marqué à la His-Avi Tag,Biotin.

Séquence: Asp19-Ser258

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 0.24μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Autre désignation: B7-H2 (ICOSLG Produits)

Synonymes: ICOSLG Protein, AU044799 Protein, B7-H2 Protein, B7RP-1 Protein, B7h Protein, BG071784 Protein, GI50 Protein, GL50 Protein, GL50-B Protein, ICOS-L Protein, Icoslg Protein, LICOS Protein, Ly115l Protein, mKIAA0653 Protein, B7H2 Protein, B7RP1 Protein, CD275 Protein, ICOSL Protein, Icosl Protein, RGD1562791 Protein, inducible T-cell costimulator ligand Protein, icos ligand Protein, inducible T-cell co-stimulator ligand Protein, ICOSLG Protein, Icosl Protein, Icoslg Protein

Sujet: CD275, B7H2, B7-H2, B7RP-1, GL50, ICOSL, ICOS-L, ICOSLG, B7RP1, LICOS, ICOS ligand, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50,B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.

Poids moléculaire: 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation