BTLA Protein (AA 31-150) (His-Avi Tag)
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- Antigène Voir toutes BTLA Protéines
- BTLA (B and T Lymphocyte Associated (BTLA))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 31-150
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette BTLA protéine est marqué à la His-Avi Tag.
- Fonction
- Human BTLA Protein
- Séquence
- Lys31-Ser150
- Attributs du produit
- Recombinant Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human BTLA, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. The affinity constant of 0.99nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Discover our top product BTLA Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- BTLA (B and T Lymphocyte Associated (BTLA))
- Autre désignation
- BTLA (BTLA Produits)
- Synonymes
- BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
- Sujet
- B- and T-lymphocyte attenuator (BTLA, CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
- Poids moléculaire
- 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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