TNFSF8
(Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
Type de proteíne
Recombinant
Attributs du protein
AA 63-234
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFSF8 protéine est marqué à la His tag.
Fonction
Human CD30 Ligand/TNFSF8 Protein
Séquence
Gln63-Asp234
Attributs du produit
Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln63-Asp234.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
TNFSF8
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
Crystallography grade
TNFSF8
Origine: Souris
Hôte: Tobacco (Nicotiana tabacum)
Recombinant
≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFSF8
(Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein
Sujet
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Poids moléculaire
20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.