CD3E & CD3G (AA 23-120) protein (His tag,Biotin)

N° du produit ABIN7274145

Détail du produit

Antigène: CD3E & CD3G

Type de proteíne: Recombinant

Attributs du protein: AA 23-120

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His tag,Biotin

Fonction: Biotinylated Human CD3E & CD3G/CD3 epsilon & CD3 gamma Protein (Primary Amine Labeling)

Séquence: Asp23-Glu120 (CD3E) & Gln23-Asn106 (CD3G)

Specificité: Uni-Prot: P07766 (CD3E), P09693 (CD3G)

Attributs du produit: Recombinant Biotinylated Human CD3E & CD3G/CD3 epsilon & CD3 gamma Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G).

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, His Tag with the EC50 of 0.43μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD3E & CD3G

Autre désignation: CD3E&CD3G

Sujet: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Poids moléculaire: 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

UniProt: P07766