CD58 Protein (CD58) (AA 29-215) (Fc Tag)

N° du produit ABIN7274195

Détail du produit

Antigène: CD58

Type de proteíne: Recombinant

Attributs du protein: AA 29-215

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD58 protéine est marqué à la Fc Tag.

Fonction: Human CD58 Protein

Séquence: Phe29-Arg215

Attributs du produit: Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 9.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD58

Autre désignation: CD58 (CD58 Produits)

Synonymes: LFA-3 Protein, LFA3 Protein, ag3 Protein, CD58 molecule Protein, CD58 Protein

Sujet: Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes, therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.

Poids moléculaire: 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.