CD74 Protein (AA 73-232) (His tag)

N° du produit ABIN7274222

Détail du produit

Antigène: CD74 (HLA-DR-gamma (CD74))

Type de proteíne: Recombinant

Attributs du protein: AA 73-232

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD74 protéine est marqué à la His tag.

Fonction: Human CD74/DHLAG Protein

Séquence: Gln73-Met232

Attributs du produit: Recombinant Human CD74/DHLAG Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln73-Met232.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD74 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74 (CD74 Produits)

Synonymes: DHLAG Protein, HLADG Protein, II Protein, Ia-GAMMA Protein, CLIP Protein, Ii Protein, INVG34 Protein, Iclp-1 Protein, iclp1 Protein, wu:fb11f09 Protein, wu:fb12a01 Protein, wu:fb13d06 Protein, wu:fk94e07 Protein, Iclp-2 Protein, cd74 Protein, iclp2 Protein, zgc:123161 Protein, CD74 molecule Protein, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Protein, CD74 molecule, major histocompatibility complex, class II invariant chain a Protein, CD74 molecule, major histocompatibility complex, class II invariant chain b Protein, CD74 Protein, Cd74 Protein, cd74a Protein, cd74b Protein

Sujet: CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.

Poids moléculaire: 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_004346

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints