DDR1 Protein (AA 21-417) (His tag)

N° du produit ABIN7274460

Détail du produit

Antigène: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Type de proteíne: Recombinant

Attributs du protein: AA 21-417

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette DDR1 protéine est marqué à la His tag.

Fonction: Human DDR1 Protein

Séquence: Asp21-Ala417

Attributs du produit: Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DDR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Autre désignation: DDR1 (DDR1 Produits)

Synonymes: CG11573 Protein, CG13984 Protein, CG13985 Protein, CG33531 Protein, CG9488 Protein, CG9490 Protein, CT33540 Protein, DDR Protein, Dmel\\CG33531 Protein, Dmel_CG13984 Protein, 6030432F18 Protein, AI323681 Protein, CD167a Protein, Cak Protein, Nep Protein, PTK3A Protein, CAK Protein, CD167 Protein, EDDR1 Protein, HGK2 Protein, MCK10 Protein, NEP Protein, NTRK4 Protein, PTK3 Protein, RTK6 Protein, TRKE Protein, Drd1 Protein, PTK3D Protein, Discoidin domain receptor Protein, discoidin domain receptor family, member 1 Protein, discoidin domain receptor tyrosine kinase 1 Protein, Ddr Protein, Ddr1 Protein, DDR1 Protein

Sujet: Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways, however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

Poids moléculaire: 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.

Pathways: Signalisation RTK, Smooth Muscle Cell Migration