EGF Protein (AA 971-1023)

N° du produit ABIN7274522

Protéine Recombinant EGF exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour EGF Protein (AA 971-1023) (ABIN7274522)

Antigène: EGF (Epidermal Growth Factor (EGF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Détail du produit

Attributs du protein: AA 971-1023

Fonction: Human EGF Protein

Séquence: Asn971-Arg1023

Attributs du produit: Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 0.2 EU per μg by the LAL method.

Biological Activity Comment: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).,Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test).,Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.1 - 0.2 ng/mL (QC Test).,The purity of Human EGF is greater than 95% as determined by SEC-HPLC.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

Sujet: The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Poids moléculaire: 5.6 kDa same as Bis-Tris PAGE result.

UniProt: P01133

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation