Fc epsilon RI/FCER1A Protein (AA 26-205) (Fc Tag)
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- Antigène Voir toutes Fc epsilon RI/FCER1A (FCER1A) Protéines
- Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 26-205
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette Fc epsilon RI/FCER1A protéine est marqué à la Fc Tag.
- Fonction
- Human Fc epsilon RI alpha/FCER1a Protein
- Séquence
- Val26-Gln205
- Attributs du produit
- Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val26-Gln205.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))
- Autre désignation
- Fc epsilon RI alpha (FCER1A Produits)
- Synonymes
- FCER1A Protein, FCE1A Protein, FcERI Protein, Iger01 Protein, RATIGER01 Protein, Fce1a Protein, Fcr-5 Protein, fcepsilonri Protein, Fc fragment of IgE receptor Ia Protein, Fc receptor, IgE, high affinity I, alpha polypeptide Protein, FCER1A Protein, Fcer1a Protein
- Sujet
- Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
- Poids moléculaire
- 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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