FGF21 Protein (mFc-Avi Tag)
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- Antigène Voir toutes FGF21 Protéines
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette FGF21 protéine est marqué à la mFc-Avi Tag.
- Séquence
- His29-Ser209
- Pureté
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Autre désignation
- FGF21 (FGF21 Produits)
- Synonymes
- FGF21 Protein, fibroblast growth factor 21 Protein, FGF21 Protein, fgf21 Protein, Fgf21 Protein
- Sujet
- UNQ3115, PRO10196, FGF-21, FGF21,Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
- Poids moléculaire
- 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
- Pathways
- Signalisation RTK
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