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FGF21 Protein (mFc-Avi Tag)

FGF21 Origine: Humain Hôte: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
N° du produit ABIN7274635
  • Antigène Voir toutes FGF21 Protéines
    FGF21 (Fibroblast Growth Factor 21 (FGF21))
    Type de proteíne
    Recombinant
    Origine
    • 19
    • 9
    • 5
    • 2
    • 1
    • 1
    • 1
    Humain
    Source
    • 22
    • 12
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette FGF21 protéine est marqué à la mFc-Avi Tag.
    Séquence
    His29-Ser209
    Pureté
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    FGF21 (Fibroblast Growth Factor 21 (FGF21))
    Autre désignation
    FGF21 (FGF21 Produits)
    Synonymes
    FGF21 Protein, fibroblast growth factor 21 Protein, FGF21 Protein, fgf21 Protein, Fgf21 Protein
    Sujet
    UNQ3115, PRO10196, FGF-21, FGF21,Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
    Poids moléculaire
    46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
    Pathways
    Signalisation RTK
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