FLT3 Protein (AA 28-541) (Fc Tag)

N° du produit ABIN7274655

Détail du produit

Antigène: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Type de proteíne: Recombinant

Attributs du protein: AA 28-541

Origine: Cynomolgus

Source: HEK-293 Cells

Purification/Conjugué: Cette FLT3 protéine est marqué à la Fc Tag.

Fonction: Cynomolgus FLT3/Flk-2 Protein

Séquence: Gln28-Ser541

Attributs du produit: Recombinant Cynomolgus FLT3/Flk-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln28-Ser541.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FLT3 Ligand, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus FLT3, hFc Tag with the EC50 of 18ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Autre désignation: FLT3 (FLT3 Produits)

Synonymes: FLT3 Protein, B230315G04 Protein, CD135 Protein, Flk-2 Protein, Flk2 Protein, Flt-3 Protein, Ly72 Protein, wmfl Protein, FLK-2 Protein, FLK2 Protein, STK1 Protein, fms related tyrosine kinase 3 Protein, FMS-like tyrosine kinase 3 Protein, fms-related tyrosine kinase 3 Protein, FLT3 Protein, Flt3 Protein

Sujet: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Poids moléculaire: 84.58 kDa. Due to glycosylation, the protein migrates to 160-180 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5VQ48

Pathways: Signalisation RTK