GFRAL Protein (His-Avi Tag)

N° du produit ABIN7274739

Détail du produit

Antigène: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette GFRAL protéine est marqué à la His-Avi Tag.

Séquence: Gln20-Glu350

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Autre désignation: GFRAL (GFRAL Produits)

Synonymes: RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein

Sujet: GFR alpha-like, GFRAL, GRAL, C6orf144,GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Poids moléculaire: 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.