ICOS Protein (Cys136Ser-Mutant, Cys137Ser-Mutant) (His tag)
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- Antigène Voir toutes ICOS Protéines
- ICOS (Inducible T-Cell Co-Stimulator (ICOS))
- Type de proteíne
- Recombinant
- Attributs du protein
- Cys136Ser-Mutant, Cys137Ser-Mutant
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette ICOS protéine est marqué à la His tag.
- Fonction
- Human ICOS/CD278 (C136S, C137S) Protein
- Séquence
- Glu21-Phe141 (C136S,C137S)
- Attributs du produit
- Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ICOS (C136S,C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product ICOS Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- ICOS (Inducible T-Cell Co-Stimulator (ICOS))
- Autre désignation
- ICOS (ICOS Produits)
- Synonymes
- ICOS Protein, AILIM Protein, CCLP Protein, CRP-1 Protein, H4 Protein, Ly115 Protein, CD278 Protein, CVID1 Protein, Ailim Protein, inducible T-cell costimulator Protein, inducible T cell co-stimulator Protein, inducible T-cell co-stimulator Protein, ICOS Protein, LOC100232284 Protein, Icos Protein
- Sujet
- Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
- Poids moléculaire
- 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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