IL-10 Protein (AA 19-178)
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- Antigène Voir toutes IL-10 (IL10) Protéines
- IL-10 (IL10) (Interleukin 10 (IL10))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 19-178
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Origine
- Humain
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Source
- HEK-293 Cells
- Fonction
- Human IL-10 Protein
- Séquence
- Ser19-Asn178
- Attributs du produit
- Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA. The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 10 (IL10) protein (His-Avi Tag)
IL10 Origine: Humain Hôte: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 10 (IL10) (AA 19-178) (Active) proteinIL10 Origine: Humain Hôte: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 10 (IL10) protein (His-Avi Tag,Biotin)IL10 Origine: Humain Hôte: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 10 (IL10) (Active) proteinIL10 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 10 (IL10) (AA 22-178) protein (His tag)IL10 Origine: Hamster Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 10 (IL10) (AA 1-178) protein (GST tag)IL10 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 10 (IL10) (AA 74-178) protein (GST tag)IL10 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 10 (IL10) proteinIL10 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Interleukin 10 (IL10) (AA 19-178) protein (His tag)Crystallography grade IL10 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL-10 (IL10) (Interleukin 10 (IL10))
- Autre désignation
- IL-10 (IL10 Produits)
- Synonymes
- CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein
- Sujet
- Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
- Poids moléculaire
- 18.8 kDa same as Tris-Bis PAGE result.
- UniProt
- P22301
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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