IL-15/IL-15R (AA 31-108) protein (His tag,Biotin)

N° du produit ABIN7274890

Détail du produit

Antigène: IL-15/IL-15R

Type de proteíne: Recombinant

Attributs du protein: AA 31-108

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His tag,Biotin

Fonction: Biotinylated Human IL-15RA & IL-15 Protein (Primary Amine Labeling)

Séquence: Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)

Specificité: Uni-Prot: Q13261-1 (IL-15RA), P40933-1 (IL-15)

Attributs du produit: Recombinant Biotinylated Human IL-15RA & IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15).

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-2 R beta&IL-2 R gamma, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-15RA&IL-15, His Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-15/IL-15R

Autre désignation: IL-15RA&IL-15

Sujet: Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Poids moléculaire: 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Tris-Bis PAGE result.