IL1R2 Protein (AA 14-343) (Fc Tag)
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- Antigène Voir toutes IL1R2 Protéines
- IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 14-343
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette IL1R2 protéine est marqué à la Fc Tag.
- Fonction
- Human IL-1R2/IL-1 RII/CD121b Protein
- Séquence
- Phe14-Glu343
- Attributs du produit
- Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe14-Glu343.
- Pureté
- > 95 % as determined by Tris-Bis PAGE
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA. The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))
- Autre désignation
- IL-1R2 (IL1R2 Produits)
- Synonymes
- CD121b Protein, CDw121b Protein, IL-1R-2 Protein, IL-1RT-2 Protein, IL-1RT2 Protein, IL1R2c Protein, IL1RB Protein, Il1r-2 Protein, IL-1R2 Protein, interleukin 1 receptor type 2 Protein, interleukin 1 receptor, type II Protein, interleukin-like Protein, IL1R2 Protein, Il1r2 Protein, KK34 Protein
- Sujet
- The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface.
- Poids moléculaire
- 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- NP_004624
- Pathways
- Signalisation NF-kappaB
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