IL-10RA Protein (AA 22-235) (His-Avi Tag)

N° du produit ABIN7274906

Détail du produit

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Type de proteíne: Recombinant

Attributs du protein: AA 22-235

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL-10RA protéine est marqué à la His-Avi Tag.

Fonction: Human IL-10 R alpha Protein

Séquence: His22-Asn235

Attributs du produit: Recombinant Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-Asn235.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Autre désignation: IL-10R alpha (IL10RA Produits)

Synonymes: IL10R1 Protein, IL10RA Protein, CD210 Protein, CD210a Protein, CDW210A Protein, HIL-10R Protein, IL-10R1 Protein, IL10R Protein, AW553859 Protein, CDw210 Protein, CDw210a Protein, Il10r Protein, mIL-10R Protein, interleukin 10 receptor subunit alpha Protein, interleukin 10 receptor, alpha Protein, IL10RA Protein, Il10ra Protein

Sujet: The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

Poids moléculaire: 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.

UniProt: Q13651

Pathways: Growth Factor Binding