IL-31 Protein (AA 27-163) (His tag)

N° du produit ABIN7274936

Détail du produit

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Type de proteíne: Recombinant

Attributs du protein: AA 27-163

Origine: Cynomolgus

Source: HEK-293 Cells

Purification/Conjugué: Cette IL-31 protéine est marqué à la His tag.

Fonction: Cynomolgus IL-31 Protein

Séquence: Leu27-Thr163

Attributs du produit: Recombinant Cynomolgus IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu27-Thr163.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.715 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Autre désignation: IL-31 (IL31 Produits)

Synonymes: IL-31 Protein, 1700013B14Rik Protein, IL31 Protein, interleukin 31 Protein, IL31 Protein, Il31 Protein

Sujet: Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.

Poids moléculaire: 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.