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IL-15 Protéine

IL15 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
N° du produit ABIN7274953
  • Antigène Voir toutes IL-15 (IL15) Protéines
    IL-15 (IL15) (Interleukin 15 (IL15))
    Type de proteíne
    Recombinant
    Origine
    • 22
    • 8
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 35
    • 10
    • 10
    • 2
    • 2
    Escherichia coli (E. coli)
    Séquence
    Asn49-Ser162
    Pureté
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by SEC-HPLC
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human IL-15 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 12.1ng/ml determined by ELISA. The affinity constant of 0.056 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl ( pH 8.0). Normally 5 % trehalose is added as protectant before lyophilization.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    IL-15 (IL15) (Interleukin 15 (IL15))
    Autre désignation
    IL-15 (IL15 Produits)
    Synonymes
    IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
    Sujet
    L15, IL-15, IL-15MGC9721, Interleukin-15,Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
    Poids moléculaire
    12.8 kDa same as Tris-Bis PAGE result.
    UniProt
    P40933
    Pathways
    Signalistation JAK/STAT, Glycosaminoglycan Metabolic Process
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