IL23R Protein (AA 24-355) (mFc Tag)
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- Antigène Voir toutes IL23R Protéines
- IL23R (Interleukin 23 Receptor (IL23R))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 24-355
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette IL23R protéine est marqué à la mFc Tag.
- Fonction
- Human IL-23R Protein
- Séquence
- Gly24-Gly355
- Attributs du produit
- Recombinant Human IL-23R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gly24-Gly355.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. The affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- IL23R (Interleukin 23 Receptor (IL23R))
- Autre désignation
- IL-23R (IL23R Produits)
- Synonymes
- IL-23R Protein, IL23R Protein, il-23r Protein, interleukin 23 receptor Protein, IL23R Protein, il23r Protein, Il23r Protein
- Sujet
- Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade.
- Poids moléculaire
- 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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