LCAT Protein (AA 25-440) (His tag)

N° du produit ABIN7275130

Détail du produit

Antigène: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Type de proteíne: Recombinant

Attributs du protein: AA 25-440

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette LCAT protéine est marqué à la His tag.

Fonction: Human LCAT Protein

Séquence: Phe25-Glu440

Attributs du produit: Recombinant Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu440.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LCAT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Autre désignation: LCAT (LCAT Produits)

Synonymes: AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein

Sujet: Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.

Poids moléculaire: 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

UniProt: P04180

Pathways: Lipid Metabolism