LILRB2
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))
Type de proteíne
Recombinant
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette LILRB2 protéine est marqué à la His-Avi Tag,Biotin.
Séquence
Gln22-His458
Pureté
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-LILRB2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB2, His Tag with the EC50 of 72.6ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
LILRB2
Origine: Humain
Hôte: Cellules d'insectes
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Stock
4 °C,-80 °C
Stockage commentaire
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Date de péremption
12 months
Antigène
LILRB2
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))
CD85D Protein, ILT-4 Protein, ILT4 Protein, LIR-2 Protein, LIR2 Protein, MIR-10 Protein, MIR10 Protein, leukocyte immunoglobulin-like receptor subfamily A member 3 Protein, leukocyte immunoglobulin like receptor B2 Protein, LOC100433990 Protein, LILRB2 Protein
Sujet
CD85d, ILT4, ILT-4, ILT4CD85d, LILRB2, LIR2, MIR10,The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Poids moléculaire
50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.