Cette LRRC15 protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Human LRRC15/LIB Protein
Séquence
Tyr22-Gly538
Attributs du produit
Recombinant Biotinylated Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-LRRC15 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 38.3ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
LRRC15
Origine: Souris
Hôte: Cellules d'insectes
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, Crys, SDS
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Poids moléculaire
60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.