TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Type de proteíne
Recombinant
Attributs du protein
AA 29-216
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF4 protéine est marqué à la Fc Tag.
Fonction
Human OX40/TNFRSF4/CD134 Protein
Séquence
Leu29-Ala216
Attributs du produit
Recombinant Human OX40 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu29-Ala216.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human OX40 Ligand (Trimer) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.27μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Poids moléculaire
46.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.