Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag,Biotin)

N° du produit ABIN7275637

Détail du produit

Antigène: Sclerostin (SOST)

Type de proteíne: Recombinant

Attributs du protein: AA 24-213

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Sclerostin protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human SOST/Sclerostin Protein

Séquence: Gln24-Tyr213

Attributs du produit: Recombinant Biotinylated Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human SOST, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Sclerostin (SOST)

Autre désignation: SOST (SOST Produits)

Synonymes: LOC100313724 Protein, CDD Protein, VBCH Protein, 5430411E23Rik Protein, sclerostin Protein, LOC100313724 Protein, SOST Protein, Sost Protein

Sujet: SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Poids moléculaire: 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.