SIRP AlphaV2 (AA 31-369) protein (His-Avi Tag,Biotin)

N° du produit ABIN7275662

Détail du produit

Antigène: SIRP AlphaV2

Type de proteíne: Recombinant

Attributs du protein: AA 31-369

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: His-Avi Tag,Biotin

Fonction: Biotinylated Human SIRP alpha V2/CD172a Protein

Séquence: Glu31-Arg369

Attributs du produit: Recombinant Biotinylated Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human SIRP alpha V2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.11μng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: SIRP AlphaV2

Autre désignation: SIRP alpha V2

Sujet: Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Poids moléculaire: 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.