CLEC4C Protein (AA 46-213) (His tag)

N° du produit ABIN7490208

Détail du produit

Antigène: CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))

Type de proteíne: Recombinant

Attributs du protein: AA 46-213

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CLEC4C protéine est marqué à la His tag.

Fonction: Human BDCA-2 Protein

Séquence: Phe46-Ile213

Attributs du produit: Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine ( pH 8.5).

Stock: -80 °C

Stockage commentaire: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))

Autre désignation: BDCA-2 (CLEC4C Produits)

Synonymes: BDCA2 Protein, CD303 Protein, CLECSF11 Protein, CLECSF7 Protein, DLEC Protein, HECL Protein, PRO34150 Protein, Clec4c Protein, C-type lectin domain family 4 member C Protein, CLEC4C Protein, LOC100733227 Protein

Sujet: BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.

Poids moléculaire: 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.