CRISPR-Cas9 (Active) protein (NLS)

N° du produit ABIN7506276

Détail du produit

Antigène: CRISPR-Cas9

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Streptococcus pyogenes

Source: Escherichia coli (E. coli)

Purification/Conjugué: NLS

Application: Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)

Fonction: Cas9-C-NLS nuclease

Attributs du produit: Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with a C-terminal nucleic localization signal (NLS).

Purification: E. coli expression system

niveau d'endotoxine: < 0.1 eu/μg

Biological Activity Comment: 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.

Information d'application

Indications d'application: CRISPR Genome editing
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 % Glycerol PH 7.4

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détail du antigène

Antigène: CRISPR-Cas9

Autre désignation: Cas9 nuclease