CRISPR-Cas9 protein (NLS)

N° du produit ABIN7509364

Détail du produit

Antigène: CRISPR-Cas9

Type de proteíne: Recombinant

Origine: Streptococcus aureus

Source: Escherichia coli (E. coli)

Purification/Conjugué: NLS

Application: Genome Editing with Engineered Nucleases (GEEN)

Fonction: SaCas9 2NLS Nuclease

Attributs du produit: Recombinant Streptococcus aureus tag-free Cas9 nuclease expressed in an Escherichia coli with both N-terminal and C-terminal nucleic localization signal (NLS).

Purification: Escherichia coli expression system

niveau d'endotoxine: 10 EU/mg as analyzed by gel clotting method

Information d'application

Indications d'application: CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Procédure de l'essai:

Cleavage assay: A 20 uL reaction in 1xSaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 2NLS Nuclease for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, pH 7.5 (50 % glycerol).

Stock: -20 °C

Détail du antigène

Antigène: CRISPR-Cas9

Autre désignation: Cas9 nuclease