IL12 Protéine

N° du produit ABIN7536200

Protéine Recombinant IL12 exprimée dans HEK-293 Cells.

Aperçu rapide pour IL12 Protéine (ABIN7536200)

Antigène: IL12 (Interleukin 12 (IL12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Détail du produit

Fonction: Active Recombinant Mouse IL-12A&IL-12B Protein

Séquence: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA&MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

Specificité: Arg23-Ala215,Met23-Ser335

Stérilité: 0.22 μm filtered

niveau d'endotoxine: <0.1EU/μg

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA.Immobilized Mouse IL-12A&IL-12B at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.001-3.3 ng/mL.|2.Active Recombinant Mouse IL-12(Cat. RP01691)induces the mouse T cells differentiate into Th1 cells. Flow cytometry was used to detect the expression of IFN-γ. Result showed that Th1 cells were induced successfully.(Customer Feedback Data).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Stock: -20 °C,-80 °C

Stockage commentaire: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12A&IL-12B

Sujet:

Description: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.

Name: p35, Ll12a, Il-12a, IL-12p35&p40, Il-12b, Il12p40, Il-12p40,IL-12&Il12b,IL-12 p70(IL12A&IL12B)

ID gène: 16159

UniProt: P43431, P43432

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome