FCGR3B Protein (AA 17-200) (His-Avi Tag,Biotin)

N° du produit ABIN7536876

Détail du produit

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Type de proteíne: Recombinant

Attributs du protein: AA 17-200

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR3B protéine est marqué à la His-Avi Tag,Biotin.

Application: SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)

Fonction: Biotinylated human Fc gamma RIIIb / CD16b (NA1) protein

Séquence: GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNENL ISSQASSYFI DAATVDDSGE YRCQTNLSTL SDPVQLEVHV GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL HKVTYLQNGK DRKYFHHNSD FHIPKATLKD SGSYFCRGLV GSKNVSSETV NITITQGLAV STISGGGLND IFEAQKIEWH EGGGENLYFQ SGGHHHHHHH HHH

Specificité: IgG

Attributs du produit: The sequence of the extracellular domain of human CD16b (Gly 17-Ser 200) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Allotype: NA1

Purification: Nickel and SEC

Pureté: > 95 % by SEC-HPLC

niveau d'endotoxine: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIb / CD16b (NA1) protein, immobilized on a CM5 chip via an anti-His antibody, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 5.0 μM.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Date de péremption: 12 months

Détail du antigène

Antigène: FCGR3B (Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))

Autre désignation: CD16b (FCGR3B Produits)

Synonymes: CD16 Protein, CD16b Protein, FCG3 Protein, FCGR3 Protein, FCR-10 Protein, FCRIII Protein, FCRIIIb Protein, FcRIII Protein, Fc fragment of IgG receptor IIIb Protein, Fc receptor, IgG, low affinity III Protein, Fc fragment of IgG receptor IIIa Protein, Fc-gamma RIII Protein, FCGR3B Protein, Fcgr3 Protein, FCGR3A Protein, FCGR3 Protein

Sujet: CD16B, FCGR3B, FCGRIIIB, FCR3B, FCRIIIB, IGFR3B, IGFRIIIB
Background: Low affinity immunoglobulin gamma Fc receptor IIIb, also known as FcγRIIIb or CD16b, is a glycosylphosphatidylinositol (GPI) anchored glycoprotein. CD16b is a member of the immunoglobulin superfamily and is expressed on exclusively on neutrophils. CD16b binds monomeric IgG with low affinity but is efficient at binding immune complexes and acts as a decoy with no known signaling mechanism. CD16b is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain and a GPI membrane anchor with no cytoplasmic tail. The product provided only contains the extracellular portion of CD16b. CD16b has two allotypic variants, referred to as human neutrophil antigen 1 (NA1 or HNA1a) and 2 (NA2 or HNA1b). The allotypes have differing affinities to human IgG1 and IgG3 with the NA1 form capable of better ingestion of IgG1 or opsonized IgG3 particles than NA2.

Poids moléculaire: 25.4 kDa

UniProt: O75015