SHP1 Protéine

N° du produit ABIN934896

Détail du produit

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Séquence: MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY

Attributs du produit: Purified recombinant Human SHP1 protein
Expression System: E.coli
Bioactivity: >5,000units/mg of SHP-1
Molecular weight on SDS-PAGE will appear higher.

Pureté: > 95 % pure

niveau d'endotoxine: < 1.0 EU per µg of protein (determined by LAL method)

Information d'application

Indications d'application: SHP1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Commentaires:

One unit will hydrolyze 1 nM of p-nitrophenyl- phosphatate(pNPP) per minute at pH 7.4 at 37 °C using 10 mM of substrate.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 25 mM Tris-HCl, pH7.5, containing 2 mM beta-mercaptoethanol, 1 mM EDTA.1 mMDTT, and 20 % glycerol.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Stockage commentaire: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Détail du antigène

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Autre désignation: SHP1 (PTPN6 Produits)

Synonymes: HCPH Protein, HPTP1C Protein, PTP-1C Protein, SH-PTP1 Protein, SHP-1 Protein, SHP-1L Protein, SHP1 Protein, DRPLA Protein, Hcph Protein, Ptp1C Protein, me Protein, motheaten Protein, Ptph6 Protein, Shp-1 Protein, fj22f05 Protein, wu:fj22f05 Protein, zgc:55501 Protein, ptpn6 Protein, protein tyrosine phosphatase, non-receptor type 6 Protein, protein tyrosine phosphatase, non-receptor type 6 L homeolog Protein, PTPN6 Protein, Ptpn6 Protein, ptpn6 Protein, ptpn6.L Protein

Sujet: The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.
Alternative Names: Protein tyrosine phosphatase non-receptor type 6 isoform 1 protein, PTPN6 protein, SHP1, SHP 1 protein, SHP-1, Tyrosine-protein phosphatase non-receptor type 6 protein, Hematopoietic cell phosphatase protein, Hematopoietic cell protein tyrosine phosphatase protein, Protein tyrosine phosphatase SHP 1 protein, SHP-1 protein, HCP protein, HCPH protein, Protein-tyrosine phosphatase 1C protein, SHP 1, SHPTP 1 protein, HCP protein, SHP 1 protein, SH PTP1 protein, 70 kda SHP1L protein protein, SHP 1L protein, SH PTP 1 protein, SH-PTP1 protein, PTP1C protein, Protein-tyrosine phosphatase SHP-1 protein, PTP 1C protein, HPTP1C protein, Protein tyrosine phosphatase 1C protein, SHP1 protein, HPTP 1C protein, SHP-1L EC 3.1.3.48 protein, PTPN6 protein, Hematopoietic cell protein-tyrosine phosphatase protein, Protein tyrosine phosphatase SHP1 protein, Tyrosine protein phosphatase non receptor type 6, SHPTP1 protein, SHP1L protein, PTPN 6 protein, PTP-1C protein, EC 3.1.3.48 protein

Poids moléculaire: 34.3 kDa (300 AA)

Pathways: Signalistation JAK/STAT, TCR Signaling, Signalisation TLR, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling