EGF Protéine
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- Antigène Voir toutes EGF Protéines
- EGF (Epidermal Growth Factor (EGF))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Séquence
- MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR
- Attributs du produit
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Purified recombinant Human EGF protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 0.89 ng/mL . Measured in a cell proliferation assay using mouse Balb3T3 cell.
Molecular weight on SDS-PAGE will appear higher. - Pureté
- > 95 % pure
- Top Product
- Discover our top product EGF Protéine
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- Indications d'application
- EGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Procédure de l'essai
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Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/mL - 1 µg/mL Detection method: BrdU assay
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in PBS, pH 7.4.
- Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles.
- Stock
- RT/-20 °C
- Stockage commentaire
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Antigène
- EGF (Epidermal Growth Factor (EGF))
- Autre désignation
- EGF (EGF Produits)
- Synonymes
- HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein
- Sujet
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Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: Epidermal growth factor protein, Urogastrone protein, Epidermal growth factor protein, epidermal growth factor (beta-urogastrone), EGF protein, Pro-epidermal growth factor protein - Poids moléculaire
- 6.3 kDa (54 AA)
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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