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Chèvre anti-Humain IgG (F(ab')2 Region) Anticorps - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
N° du produit ABIN101607
  • Antigène Tous les produits IgG
    IgG
    Épitope
    • 2272
    • 457
    • 362
    • 188
    • 130
    • 123
    • 93
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    F(ab')2 Region
    Reactivité
    • 673
    • 658
    • 556
    • 430
    • 366
    • 219
    • 199
    • 144
    • 134
    • 122
    • 109
    • 93
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    • 3
    • 3
    • 3
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    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 1660
    • 1418
    • 532
    • 217
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3307
    • 194
    • 1
    Polyclonal
    Application
    • 1777
    • 1713
    • 1689
    • 1443
    • 934
    • 910
    • 883
    • 463
    • 234
    • 115
    • 115
    • 100
    • 92
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    • 50
    • 50
    • 32
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgG F(ab')2
    Attributs du produit
    Anti-Human IgG F(ab')2 fragment antibody generated in goat detects specifically Human IgG F(ab')2 fragment. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Human IgG F(ab')2 fragment antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Stérilité
    Sterile filtered
    Immunogène

    Immunogen: Human IgG F(ab')2 fragment was produced by repeated immunization with Human IgG F(ab')2 fragment in goat.

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgG F(ab')2 fragment is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:14,000 - 1:30,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Goat Anti-Human IgG Fab2 Antibody, Goat Anti-Human IgG F(ab')2 Antibody

    Background: Anti-Human IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG F(ab')2 fragment antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

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