Chèvre anti-Rat IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Fc Region
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Reactivité
- Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- IgG F(c)
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Stérilité
- Sterile filtered
- Immunogène
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Immunogen: Rat IgG F(c) fragment
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Rat F(c) IgG Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:5,000
Western Blot Dilution: 1:500 - 1:2,500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: Goat Anti-Rat IgG F(c) alkaline phosphatase Conjugated Antibody, Goat Anti-Rat IgG F(c) fragment Antibody alk phos Conjugation
Background: Anti-Rat IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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