Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN102259

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG (H&L)

Réactivité croisée: Mouton

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Stérilité: Sterile filtered

Immunogène:

Immunogen: Sheep IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Secondary antibody conjugated to alkaline phosphatase is available in a variety of formats. Conjugated Secondary Antibody is suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:1,000 - 1:5,000

Western Blot Dilution: 1:400 - 1:1,600

Commentaires:

When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Rabbit Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody alk phos Conjugation

Background: Anti-Sheep IgG (H&L) Alkaline Phosphatase Antibody generated in rabbit detects reactivity to sheep IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG is conjugated to Alkaline Phosphatase.