Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN1044051

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Purification: Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads

Stérilité: Sterile filtered

Immunogène:

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200-1:1,000

Application Note: This product has been assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:2,000-1:10,000

Western Blot Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody alk phos Conjugation

Background: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.