Based on immunoelectrophoresis, this antibody reacts with: heavy (epsilon) chains on human IgE (epsilon chain)
Attributs du produit
Goat anti-human IgE (ε chain) - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human IgE (ε chain) in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgE (ε chain).
For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v).
Conseil sur la manipulation
Product is photosensitive and should be protected from light. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Stock
4 °C/-20 °C
Stockage commentaire
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.