Chèvre anti-Souris IgG2b (Heavy Chain) Anticorps (Atto 532) - Preadsorbed

N° du produit ABIN1118498

Détail du produit

Antigène: IgG2b

Épitope: Heavy Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Atto 532

Application: Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Réactivité croisée: Souris

Attributs du produit: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG2b heavy chain

Immunogen Type: Native Protein

Isotype: IgG2b

Labeling Ratio: 3.0

Information d'application

Indications d'application:

Application Note: Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Flow Cytometry Dilution: 1:500 - 1:2,500

Western Blot Dilution: 1:4,000 - 1:20,000

IF Microscopy Dilution: >1:5,000

Commentaires:

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG2b

Abstract: IgG2b Produits

Classe de substances: Antibody

Sujet:

Synonyms: goat anti-MOUSE IgG2b Antibody ATTO532 Conjugation, goat anti-Mouse IgG2b (gamma 2b) ATTO 532 Conjugated Antibody

Background: Anti-Mouse IgG2b ATTO 532 Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.