Chèvre anti-Souris IgG1 (Heavy Chain) Anticorps (Atto 532) - Preadsorbed
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- Antigène Tous les produits IgG1
- IgG1
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Épitope
- Heavy Chain
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Reactivité
- Souris
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Atto 532
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Specificité
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Réactivité croisée
- Souris
- Attributs du produit
- ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
- Isotype
- IgG1
- Labeling Ratio
- 2.5
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- Indications d'application
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Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Commentaires
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.
Product is photosensitive and should be protected from light. - Stock
- RT,4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- IgG1
- Abstract
- IgG1 Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: Goat anti-mouse IgG1 antibody ATTO488 conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 488 conjugated antibody
Background: Anti-Mouse IgG1 ATTO 532 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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