Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Concentration
2.0 mg/mL
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
Biohazard Informations: This product contains thimerosal which is hazardous.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.