Lapin anti-Humain IgG (Chain gamma) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Chain gamma
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
- Human IgG gamma chains.
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Goat anti-Human IgG (F(ab')2 Region) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
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Mouse anti-Human IgG (Heavy & Light Chain) antibodyELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated
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- Indications d'application
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other phosphatase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 g of Human IgG in astandard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutesat room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA IgG and Protease free) and 0.01 % (w/v) Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Conseil sur la manipulation
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Stock
- 4 °C
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
- Synonyms: Human Immunoglobulin G
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