Chèvre anti-Souris IgG, IgM (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN129738

Détail du produit

Antigène: IgG, IgM

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: TRITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Specificité: IgG + IgM (H&L)

Attributs du produit: Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Mouse IgM antibody is suitable for immunosassays including western blot and ELISA.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG and IgM whole molecules

Isotype: IgG

Labeling Ratio: 3.3

Information d'application

Indications d'application:

Application Note: Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgG and IgM antibody is suitable for immunoassays including western blot and ELISA.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG, IgM

Autre désignation: IgG + IgM (IgG, IgM Produits)

Classe de substances: Antibody

Sujet:

Synonyms: Goat Anti-Mouse IgG + IgM Antibody Fluorescein Conjugated Pre-Adsorbed, Goat Anti-Mouse IgG/IgM Antibody Fluorescein Conjugated

Background: Anti-Mouse IgG + IgM Rhodamine Antibody generated in Goat detects reactivity to Mouse IgG and IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Rhodamine Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.